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KMID : 0366119770050010036
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Korean Journal of Applied Microbiology & Bioengineering
1977 Volume.5 No. 1 p.36 ~ p.45
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Biological Active Substance Produced by a Strain of Streptomyces sp
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Abstract
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A piscicidal substance was isolated from the culture medium of Streptomyces umbrosus by avicel column chromatography and avicel thin layer chromatography after extration with chroloform. Bluegreen fluorescence was emitted under UV irradiation. Factors which govern toxin production and nutrition requirement for high toxin titres were observed. Nutritional uptake for toxin production was not corresponded with that for cell growth. Alanine, valine, serine asparagine, arginine, histidine, urea and sodium nitrate as a carbon source and glucose, mannose, rhamnose, xylose, arabitol and starch as a carbon source were recognized as a favorable nutrient for high toxin production. Magnesium was essential factor whereas vitamins were not effective. Most of toxin was formed simultaneously with cell growth in esponential phase. Maximal production was observed for six days culture at 30 C. Tissues of gill, kidney and pnacreas in Cyprinus carpio were denatured extreamly after treating with the substance. Atrophied nucleous, indented membrane and degradated cytoplasm with necrotic affectness were noted on each tissue. The chemical formula of the substance was designated as C_(38)H_(66)NO₄.
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